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| Lorenson,M.S.; Miani,M.; Guizzo,J.A.; Barasoul,B.; Martínez-Martínez,S.; Rodríguez-Ferri,E.F.; Gutiérrez-Martín,C.B.; Kreutz,L.C.; Frandoloso,R.. |
| ABSTRACT Glässer's disease is an emergent bacterial disease that affects swine husbandries worldwide causing important economic losses. The aetiological agent, Haemophilus parasuis, is currently divided in fifteen serovars but an increasing number of non-typeable serovars have been reported. Indirect hemagglutination (IHA) is indicated as a serotyping method for H. parasuis. In the present study, we describe an additional step that aims to work around a possible obstacle in the original protocol that may compromise the outcome of this assay. We observed that the choice of anticoagulant for blood collection influences and/or impairs spontaneous adsorption of H. parasuis antigens on sheep red blood cells (SRBCs). However, regardless of the anticoagulant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Haemophilus parasuis; Diagnosis; Tannic acid; Serotyping; Indirect hemagglutination. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000100015 |
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| Pavan,T.R.; Di Domenico,J.; Kirsten,K.S.; Nied,C.O.; Frandoloso,R.; Kreutz,L.C.. |
| Adjuvants are essential to boost the immune response to inoculated antigen and play a central role in vaccine development. In this study, we investigated the efficacy of several adjuvants in the production of anti-bovine serum albumin (BSA) antibodies in silver catfish. Two hundred and seventy juvenile silver catfish (60–80 g) of both sexes were intraperitoneally vaccinated with BSA (200 µg/fish) alone or mixed to the following adjuvants: Freund’s complete adjuvant (FCA), Freund’s incomplete adjuvant (FIA), aluminum hydroxide (AlOH), Montanide, four types of cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) and three concentrations of β-glucan, and the immune enhancing property was evaluated by measuring anti-BSA antibodies in blood samples at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vaccination; Fish; Immune response; CpGs ODN; Β-glucan. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800606 |
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